The Crystal Structure of Six Transmembrane Antigen of the Prostate 4 (Steap4), a Ferri/Cuprireductase, Suggests a Novel Inter-Domain Flavin Binding Site
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چکیده
Background: In-depth biochemical and structural analyses of Steap4 are lacking. Results: Crystallographic and kinetic characterization of Steap4 metalloreductase and flavin dependent NADPH oxidase activities are reported. Conclusion: Physiologically relevant ferric/cupric reductase and flavin dependent NADPH oxidase activities are observed at neutral and endosomal pH; the N-terminal tail is dispensable for these activities. Significance: Metalloreductase activity should be considered in Steap4 associated insulin resistance.
منابع مشابه
The crystal structure of six-transmembrane epithelial antigen of the prostate 4 (Steap4), a ferri/cuprireductase, suggests a novel interdomain flavin-binding site.
Steap4 is a cell surface metalloreductase linked to obesity-associated insulin resistance. Initial characterization of its cell surface metalloreductase activity has been reported, but thorough biochemical characterization of this activity is lacking. Here, we report detailed kinetic analysis of the Steap4 cell surface metalloreductase activities. Steap4 shows physiologically relevant Km values...
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تاریخ انتشار 2013